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. 2021 Sep 29;9:693410. doi: 10.3389/fcell.2021.693410

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Copyright © 2021 Hunyenyiwa, Hendee, Matus, Kyi, Mammoto and Mammoto.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TWIST1 expression in obese human subcutaneous adipose ECs is lower than lean ECs. (A) Volcano plot of differentially expressed genes, including TWIST1, in eight obese vs. seven lean adult human subcutaneous adipose samples from the GSE55200 dataset. Blue indicates significantly down-regulated genes and red indicates significantly up-regulated genes with Benjamini and Hochberg adjusted p-values < 0.05. (B) Graph showing the TWIST1 mRNA levels in ECs isolated from lean (BMI < 30) vs. obese (BMI > 30) human adipose tissues (n = 9–10, mean ± SEM, *p < 0.05). The numbers along the dots correspond to the clone numbers in Table 1. (C) Immunoblots showing the TWIST1 and β-Actin protein levels in ECs isolated from lean (BMI < 30) vs. obese (BMI > 30) human adipose tissues (n = 5, mean ± SEM, *p < 0.05). The numbers along the dots correspond to the clone numbers in Table 1. (D) Network showing connections among TWIST1, SLIT2, and genes from the top 30 BP GO Term categories derived from 1,302 significantly differentially expressed down and upregulated genes with Benjamini and Hochberg adjusted p-values < 0.05 from the GSE55200 dataset. Genes are color-coded by GO Term categories. Red, angiogenesis-related genes; Orange, development-related genes; Gold, ECM and cell adhesion-related genes; Dark green, cell proliferation, migration-related genes; Blue, inflammation and/or immune response-related genes; Purple, cell signaling and/or apoptosis-related genes; Gray, miscellaneous category-related genes; Black outlines, genes from the GSE55200 dataset.