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. 2021 Sep 29;9:693410. doi: 10.3389/fcell.2021.693410

FIGURE 2.

FIGURE 2

TWIST1 controls SLIT2 expression in lean vs. obese adipose ECs. (A) Graph showing SLIT2 mRNA expression in lean vs. obese human adipose ECs (n = 9–10, mean ± SEM, *p < 0.05). (B) Graph showing SLIT2 protein expression in lean vs. obese human adipose tissues (n = 9, mean ± SEM, *p < 0.05). (C) Graphs showing mRNA expression of TWIST1 and SLIT2 in lean human adipose ECs treated with TWIST1 siRNA or control siRNA with irrelevant sequences (n = 4–5, mean ± SEM, *p < 0.05). Graph showing protein expression of SLIT2 in lean human adipose ECs treated with TWIST1 siRNA or control siRNA with irrelevant sequences (n = 4, mean ± SEM, *p < 0.05). (D) Graphs showing mRNA expression of TWIST1 and SLIT2 in obese human adipose ECs treated with lentivirus overexpressing TWIST1 or control virus (n = 4, mean ± SEM, *p < 0.05). Graph showing protein expression of SLIT2 in obese human adipose ECs treated with lentivirus overexpressing TWIST1 or control virus (n = 4, mean ± SEM, *p < 0.05). The numbers along the dots correspond to the clone numbers in Table 1.