TABLE 3.

Summary of methods used to identify GLP-2R expression throughout the gastrointestinal tract.

Cell/organ	Species	Method of identification	References
Human colorectal tumors	Human	GLP-2R mRNA transcripts expressed qRT-PCR.	Koehler et al., 2015
Human colon neoplasms	Human	Immunohistochemistry.	Koehler et al., 2008
Gastric chief cells	Human	GLP-2R Fluorescence ISH. GLP-2R by western blot.	Li et al., 2017
Myenteric plexus	Human	In vitro receptor autoradiography of human intestinal tissue.	Pedersen et al., 2015
Lamina propria stromal cells	Mouse	ISH with RNAscope, Glp-2r mRNA detected. GLP2R-driven LacZ expression.	Yusta et al., 2019
Vagal afferents	Rat	GLP-2R antibody localizing GLP-2R immunoreactivity. ISH.	Nelson et al., 2007
Intestinal muscularis	Mouse	Glp-2r mRNA transcripts by RT-PCR.	Shin et al., 2005
Jejunal enteroendocrine cells	Pig	Glp-2r mRNA transcripts by qRT-PCR of laser micro-dissected tissue. In situ hybridization. Immunostaining.	Guan et al., 2006
Subepithelial myofibroblasts	Mouse Rat Mouse	Glp-2r mRNA expression by qRT-PCR. Immunohistochemistry (antibody 99077).	Orskov et al., 2005
Subepithelial myofibroblasts	Mouse Rat Mouse	Glp-2r mRNA expression by qRT-PCR. Immunohistochemistry (antibody 99077).	Orskov et al., 2005
Isolated intestinal mucosal cells	Rat	GLP-2R mRNA transcripts by RT-PCR.	Walsh et al., 2003
Lamina propria of duodenal and jejunal villi, submucosal nerve plexuses	Mouse	RNAScope in situ hybridization.	Wismann et al., 2017
Smooth muscle layer, intestine devoid of epithelium, respectively	Rat	Glp-2r mRNA expression by qRT-PCR.	Pedersen et al., 2015