TABLE 2.

MAb, PFGE, and AP-PCR subtyping of clinical and environmental isolates of L. pneumophila serogroup 6

Strain no.a	Reactivity against MAb:				PFGE type after digestion withb:			AP-PCR type with primer:				Combined type codec
	9/2	4/5d	18/2d	54/2d	SfiI	NotI	AscI	AP5	AP12	CD1	1247
1	+	+	−	+	A	NC	F	a	e	g	j	I
2	+	−	−	−	CIII	D	G	c	e	i	l	IIII
3	+	−	−	−	CI	D	G	c	e	i	l	IIII
4	+	+	−	+	A	NC	F	a	e	g	j	l
5	+	+	−	+	B	E	H	b	f	h	k	II
6	+	+	−	+	B	E	H	b	f	h	k	II
7	+	+	−	+	B	E	H	b	f	h	k	II
8	+	+	−	+	B	E	H	b	f	h	k	II
9	+	+	−	+	B	E	H	b	f	h	k	II
10	+	−	−	−	CI	D	G	c	e	i	l	IIII
11	+	−	−	−	CV	D	G	d	e	i	l	IIIII
12	+	−	−	−	CI	D	G	c	e	i	l	IIII
13	+	−	−	−	CV	D	G	d	e	i	l	IIIII
14	+	−	−	−	CII	D	G	c	e	i	l	IIII
15	+	−	−	−	CIV	D	G	d	e	i	l	IIIII
16	+	+	−	+	A	NC	F	a	e	g	j	I
17	+	+	−	+	A	NC	F	a	e	g	j	I
18	+	−	−	−	CIV	D	G	d	e	i	l	IIIII
19	+	−	−	−	CII	D	G	c	e	i	l	IIII
Chicago 2	+	+	−	+
Dresden	+	−	−	−

^aStrains were numbered as in Table 1. 

^bIndistinguishable patterns are indicated with the same letter code. Minor differences (<20%) within a single PFGE type are indicated by superscript roman numerals; NC, genomic DNA is not cut by the enzyme. 

^cObtained by combining MAb, PFGE, and AP-PCR types; strains with >80% and 90% similarities at the macrorestriction and AP-PCR levels, respectively, were considered to belong to a single type. Superscript roman numerals indicate minor differences within a single type. 

^dMAbs that recognize monoclonal subgroups of serogroup 6.