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. 2021 Oct 5;37(1):109777. doi: 10.1016/j.celrep.2021.109777

Figure 6.

Figure 6

The absence of CYLD activity leads to reduced LUBAC retention at the TNF-RSC as well as increased Lys63-Ub and reduced Met1-Ub on receptor components

(A–C) Purification and western blot analysis of the TNFR1 complex in THP1 cells with shRNA knockdown of CYLD or mismatch control (A) and U2OS/NOD2 cell lines (B and C) stimulated with biotin-TNF as indicated.

(D) UbiCRest analysis of TNFR1 complexes purified as in (B). Samples were incubated or not with OTULIN and/or OTUD1 as indicated and analyzed by western blotting.