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. 2021 Sep;10(9):3669–3683. doi: 10.21037/tau-21-777

Figure 6.

Figure 6

MiR-124 decreases cisplatin sensitivity and cisplatin-induced necroptosis by inhibiting calpain small subunit 1 (CAPN4)-mediated CCR4-NOT transcription complex subunit 3 (CNOT3) degradation. (A) Western blot and quantitative real-time PCR (qPCR) analysis revealed that the protein and mRNA levels of CAPN4 and CNOT3 in different groups in Caki-1 and 786-O cells (miR-124, miR-124+CAPN4, miR-124+CNOT3, miR-124+ CPAN4+ CNOT3). (B) The effect of CAPN4 and/or CNOT3 on cell viability and the IC50 (half maximal inhibitory concentration) value of miR-124-overexpressed Caki-1 and 786-O cells to cisplatin were evaluated using the Cell Counting Kit-8 (CCK-8) assay. (C) The effect of CAPN4 and/or CNOT3 on the level of necroptosis in miR-124-overexpressed Caki-1 and 786-O cells to cisplatin were evaluated by flow cytometric analysis. *, P<0.05 vs. miR-124 group; #, P<0.05 vs. miR-124+ CAPN4 group.