Table 2.
Primers used in this study.
| Primer | Sequence (from 5' to 3')a |
|---|---|
| T7-C321-F | TTCCCCTGATGCTGCCTTACGCGAACGCGAAGTCCGACTCT |
| T7-C321-down-R | GTGAAACCAGTAACGTTATACGAT |
| C321-UP-F | TGCCTCTACTGCTGGCGCA |
| C321-UP-R | TCGGACTTCGCGTTCGCGTAAGGCAGCATCAGGGGAAAACCTTAT |
| SgRNA | GTGCCCGGCTTCTGACCATG |
| C321-JD-F | CATGTGCCTTCTTCCGCGTGCA |
| C321-JD-R | GGCCAGCCACGTTTCTGCGAAA |
| T7 RNApoly-F | ATGAACACGATTAACATCGCTAAGA |
| T7 RNApoly-R | TTACGCGAACGCGAAGTCCGACTCT |
| GFP-Y151TAG-F | CACAATGTA TAG ATCATGGCAGACAAACAAAAGAATGGAATCAAAG |
| GFP-Y151TAG-R | CTGCCATGAT CTA TACATTGTGTGAGTTATAGTTGTATTCCAATTTG |
| GFP-Y39TAG-F | TGCAACA TAG GGAAAACTTACCCTTAAATTTATTTGCAC |
| GFP-Y39TAG-R | GTTTTCC CTA TGTTGCATCACCTTCACCCTCTCCACTG |
| GFP-Y182TAG-F | GCAGACCAT TAG CAACAAAATACTCCAATTGGCGATGGC |
| GFP-Y182TAG-R | TTGTTG CTA ATGGTCTGCTAGTTGAACGCTTCCATCTTCAATG |
a
The underlined sequences indicate mutated codons.