Figure 11.

Arginine methylation by PRMT1 and PRMT5/MEP50 in tetra-acetylated H4 mononucleosome. Reactions were held for 30 min at 30 °C with 0.05 μM PRMT1 or PRMT5/MEP50, 5 μM [¹⁴C]SAM, and 1 μM of substrate. A, phosphor image of radiolabeled proteins after 72 h in the dark. B, Coomassie blue protein staining. [¹⁴C]BSA and a protein ladder were used in lanes 1 and 8 as a radiolabeled protein marker and a protein MW reference, respectively. [¹⁴C]BSA, [¹⁴C]-labeled bovine serum albumin; [¹⁴C]SAM, SAM-(methyl-¹⁴C); MW, molecular weight; PRMT, protein arginine methyltransferase.