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. 2021 Sep 30;26(19):5931. doi: 10.3390/molecules26195931

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of 6-shogaol on expression of prooxidant and antioxidant enzymes in cisplatin-treated mice. (A) Relative mRNA levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), 5-lipoxygenase (5-LOX), and nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4). (B) Relative mRNA levels of catalase and manganese superoxide dismutase (MnSOD). (C) Western blotting of NOX4 and MnSOD. (D) Quantification of western blots for NOX4 and MnSOD. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was chosen as an internal control. n = 8 per group of mice. * p < 0.05 versus Con. ^# p < 0.05 versus CP.