Table 1.

Pretreatment assay. Vero monolayers were treated AuNPs infected with HSV-1 at different times, and supernatants were titrated on virus replication.

AuNPs (Diameter)	Concentration (μg/mL)	HSV-1 Load in Vero Cell Supernatants (TCID50/mL)
		After Pretreatment			Non-Treated with AuNPs
		T 0.25 h	T 1 h	T 4 h
AuNPs I (10 nm)	0.295	4.30 × 105 ±1.70 × 105	1.02 × 106 ±1.02 × 106	4.30 × 105 ±1.70 × 105	5.50 × 106
	5.9	4.26 × 105 ±1.75 × 105	5.50 × 104	4.30 × 104 ±1.70 × 104	5.50 × 106
AuNPs II (16 nm)	0.295	3.09 × 106	5.50 × 106	3.09 × 106	5.50 × 106
	5.9	2.42 × 106 ±9.55 × 105	3.09 × 106	2.38 × 106 ±9.05 × 105	5.50 × 106

Data are represented as mean ± SD of two experiments performed in triplicate. Abbreviations: T, time of pretreatment (h, hours); AuNPs I, size 10 nm; AuNPs II, size 16 nm. Samples from the supernatant medium were collected at 24 h p.i.