Table 2.

BBB assay of enamide derivatives (NEA1–NEA5) using the PAMPA method.

Compounds	Bibliography [34] Pe (×10−6 cm/s)	Experimental Pe (×10−6 cm/s)	Prediction
NEA1		14.44 ± 0.35	CNS+
NEA2		13.46 ± 0.26	CNS+
NEA3		16.43 ± 0.17	CNS+
NEA4		15.54 ± 0.62	CNS+
NEA5		15.53 ± 0.44	CNS+
Progesterone	9.3	9.12 ± 0.21	CNS+
Verapamil	16.0	16.33 ± 0.44	CNS+
Piroxicam	2.5	2.37 ± 0.33	CNS±
Lomefloxacin	1.1	1.31 ± 0.51	CNS−
Dopamine	0.2	0.26 ± 0.04	CNS−

CNS+ (high BBB permeation predicted: Pe (10⁻⁶ cm/s) > 4.00). CNS− (low BBB permeation predicted: Pe (10⁻⁶ cm/s) < 2.00). CNS± (BBB permeation uncertain: Pe (10⁻⁶ cm/s) from 2.00 to 4.00).