FIG. 2.

Reverse line blot hybridization assay analyses for the detection and identification of Ehrlichia, B. burgdorferi, and Bartonella spp. in ticks. (A) Membrane carrying Ehrlichia-specific oligonucleotide probes; (B) membrane carrying B. burgdorferi genospecies-specific probes; (C) combined membrane carrying probes for Ehrlichia, B. burgdorferi, and Bartonella species. The oligonucleotide probes are attached to the membrane in the horizontal direction, and the PCR samples were applied perpendicularly in the vertical direction. The numbered lanes represent tick-derived PCR products, and the lanes marked with letters show the PCR products obtained from the positive control samples. El, Ehrlichia-like; Eca, E. canis; Ech, E. chaffeensis; Epv, E. phagocytophila variant; Hv, HGE variant; Ep, E. phagocytophila; H, HGE; Bs, B. burgdorferi sensu stricto; Bg, B. garinii; Ba, B. afzelii; Bv, B. valaisiana; Bh, B. henselae; Bq, B. quintana.