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Variables in Box Behnken design for preparation and optimization of PAX and RESV PNPs.
| Factors | Levels | |
|---|---|---|
| Independent variable | Low | High |
| X1 = Soluplus (mg·mL−1) | 10 | 30 |
| X2 = PAX-RESV (mg·mL−1) | 1 | 2.0 |
| X3 = TPGS1000 (% w/v) | 0.1 | 0.5 |
| Dependent variable | Goals | |
| Y1 = Particle size (nm) | Decrease | |
| Y2 = PDI | Decrease | |
| Y3 = Entrapment Efficiency (%) | Increase | |
