Table 3.

Standard 1× Lysate and 4× Lysate Protocol STRAT4-ERBB2 vs HER2-IHC Results^a

	STRAT4-ERBB2, No.
	1× Lysate			4× Lysate
HER2-IHC	Positive	Negative	Total	Positive	Negative	Total
Positive (3+)	33	1	34	33	1	34
Negative (0, 1+)	2	101	103	2	104	106
Total	35	102	137	35	105	140

HER2, human epidermal growth factor receptor 2; IHC, immunohistochemistry.

^aFor the 1× lysate, the overall percent agreement (OPA) was 97.8%, positive percent agreement (PPA) was 97.0%, and negative percent agreement (NPA) was 99.0%. For the 4× lysate, the OPA was 97.8%, PPA was 97.0%, and NPA was 99.0%.