Figure 2.

Effect of para-toluenesulfonamide on the distribution of cell cycle phases in NCI-H460 and A549 cells. The cells were incubated in the absence or presence of para-toluenesulfonamide at the indicated condition (A, 24 and 48 h; B, 48 h; C, 6 mM). After the treatment, the cells were harvested for the detection of cell population at different phases by flow cytometric analysis of PI staining (A), the cell apoptosis by detecting nucleosomal DNA fragmentation using Cell Death Detection ELISA^PLUS kit (B) and the detection of protein expressions of cell cycle regulators by Western blot analysis (C). Quantitative data are expressed as mean ± SEM of three independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 compared with the control.