Figure 4.

Effect of para-toluenesulfonamide on cholesterol content. NCI-H460 and A549 cells were incubated in the absence or presence of 6 mM para-toluenesulfonamide for 24 h. (A) The level of total cellular cholesterol was examined using the Cholesterol Assay Kit. (B) Plasma membrane fraction was isolated by sucrose density gradient centrifugation, and the level of cholesterol on plasma membrane was examined using the Cholesterol Assay Kit. Na⁺/K⁺ ATPase a1-subunit was used as internal control. The data are expressed as mean ± SEM of three independent determinations. * p < 0.05 compared with the control.