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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Biol Lipids. 2021 May 29;1866(9):158983. doi: 10.1016/j.bbalip.2021.158983

Fig. 2. Growth inhibition of SBF mutants by myriocin.

Fig. 2.

(A) Strains slm1Δ, WT (BY4741), WTtransformed with plasmid containing geneticin resistance (pKANr), and swi4Δ were spotted in serial dilutions and grown in synthetic minimal medium (SD) at 30ºC for 2 days (control). 15μM PHS and 0. 6μg.ml−1 myriocin were added where indicated. (B) The myriocin IC50 values for WT and swi4Δ strains were determined in SD medium. (C) Strains related to SBF (swi4Δ, swi6Δ,), MBF (mbp1Δ, nrm1Δ) M/G1 regulator (ace2Δ) and ceramide synthesis (sur2Δ, lac1Δ and tsc3Δ) were grown as in A. (D) Reversion of myriocin growth phenotype by the addition of 0.4% serine and 0.4% palmitate. (E) Strains related to G1 (cln1Δ, cln2Δ, cln3Δ), S (clb5Δ, clb6Δ) and G2/M cyclins (clb2Δ) were grown in the presence of myriocin as in A.