Skip to main content
. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Biol Lipids. 2021 May 29;1866(9):158983. doi: 10.1016/j.bbalip.2021.158983

Fig. 4. Designed assay for cell synchronization and cell cycle studies.

Fig. 4.

(A) Cells were cultivated to exponential phase in YPD medium and G1 synchronized with alpha factor. YPD was used for synchronization as swi4Δ cells do not synchronize after two hours in SD medium. Cells then were seeded in fresh minimal medium SD and three different aliquots were taken at the time points indicated to measure cell cycle stage, mRNA transcripts, and sphingolipid metabolites. (B) Transcription of CLN2, canonical target of SBF during cell cycle. WT (●) and swi4Δ cells (■). (C, D) DNA content (FACS), indicating the % of cells at each stage of the cell cycle, relative to total cells.