Fig. 2.

Mild oxidative stress induces Nrf2-regulated gene expression in Keap1^−/− cultures. A-C) Mixed astrocyte/neuronal cultures of the indicated genotypes were treated with the indicated doses of H₂O₂ for 6h after which expression of Srxn1 (A), Slc7a11 (B) and Hmox1 (C) was assessed by qRT-PCR and normalized to Gapdh. Expression levels presented herein relative to WT-control. *P < 0.05 two-way ANOVA with Tukey's post-hoc test, ^# effect of genotype on basal levels (two-way ANOVA with Sidak's post-hoc test, ^#P < 0.05 (n = 5–6). D) Mixed astrocyte/neuronal cultures of the indicated genotypes were treated with tBHQ for 6h after which gene expression was assessed as in A-C. *P < 0.05, (compared with WT-control), two-way ANOVA with Tukey's post-hoc test, (n = 4–6).