Figure 1.

Immunization schematic and T. gondii ROP4-specific antibody responses. BALB/c mice were immunized with the ROP4-rBVs vaccine through the oral and intranasal routes with blood collection at regular intervals as scheduled (A). Polyclonal T. gondii antibody collected from mice was used to assess ROP4-specificity via ELISA (B). Sera were collected 3 weeks after each immunization and T. gondii ME49 antigen-specific IgG (C) and IgA (D) antibody responses were determined by ELISA. Data are presented as mean ± SD and asterisks indicate statistical differences between groups (**P < 0.01, ***P < 0.001, ****P < 0.0001). Images were created with BioRender.com.