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The study of nonspecific adsorption between MB-ZEN-BSA and CuO NP-anti-ZEN with and without BSA (the reaction time is 10 min) (a) and the reaction time optimization between MB-ZEN-BSA (5 mg/mL, 10 μL) and CuO NP-anti-ZEN (1 mg/mL, 5 μL) (b).
