TABLE 3.
Fatty acid composition of Legionella species based on numerical analysis of FAME profilesa
| Species and cluster | Fatty acid composition (% of total)b
|
||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| i14:0 | 14:0 | i15:0 | a15:0 | 15:1ω6c | 15:0 | Alcohol 16:1ω7c | i16:1 Hc | i16:0 | 16:1ω11c | 16:1ω7c | 16:1ω5c | 16:0 | i17:1ω9c | a17:1ω9c | i17:0 | a17:0 | cyc17:0 | 17:0 | 18:0 | 19:0 | |
| L. adelaidensis, cluster 10 | 3.7 ± 0.7 | trd | tr | 15.8 ± 2.0 | 4.9 ± 0.6 | 3.9 ± 0.1 | —e | — | 17.2 ± 2.9 | — | 27.0 ± 1.7f | — | 13.0 ± 2.9 | — | — | tr | 3.4 ± 0.2 | — | tr | tr | tr |
| L. anisa, cluster 24 | 6.7 ± 1.3 | tr | tr | 24.2 ± 2.2 | tr | tr | — | — | 24.0 ± 2.0 | — | 8.0 ± 2.4 | tr | 6.9 ± 1.6 | — | — | tr | 6.4 ± 1.1 | 10.0 ± 2.9 | tr | tr | tr |
| L. birminghamensis, cluster 13 | 3.2 ± 0.5 | — | tr | 32.2 ± 4.6 | 6.8 ± 3.6 | 2.3 ± 0.9 | — | tr | 21.5 ± 1.7 | — | 14.0 ± 1.9 | — | 3.3 ± 0.9 | — | — | tr | 10.6 ± 2.0 | — | tr | — | tr |
| L. bozemanii | |||||||||||||||||||||
| Cluster 26 | 2.4 ± 0.5 | tr | tr | 26.3 ± 1.5 | tr | 2.3 ± 0.4 | — | — | 12.0 ± 1.8 | — | 6.3 ± 0.5 | tr | 11.8 ± 1.6 | — | — | tr | 10.6 ± 1.5 | 14.1 ± 1.8 | 4.8 ± 0.9 | tr | tr |
| Cluster 27 | 3.4 ± 1.0 | tr | tr | 32.8 ± 2.0 | tr | tr | — | — | 16.3 ± 2.1 | — | 6.4 ± 2.2 | tr | 7.0 ± 1.5 | tr | — | tr | 11.8 ± 3.3 | 8.7 ± 1.3 | 2.3 ± 1.1 | tr | tr |
| L. brunensis, cluster 4 | tr | tr | 4.2 ± 1.9 | 36.7 ± 2.5 | tr | tr | — | tr | 11.8 ± 1.1 | — | 5.6 ± 0.7 | — | 2.8 ± 0.4 | tr | tr | 4.4 ± 2.0 | 23.1 ± 1.9 | tr | tr | tr | — |
| L. cherrii, cluster 20 | 7.3 ± 0.5 | tr | tr | 30.1 ± 1.7 | tr | tr | — | tr | 24.4 ± 4.2 | — | 10.9 ± 2.4 | tr | 3.7 ± 1.4 | — | — | tr | 9.2 ± 1.3 | 4.7 ± 0.4 | tr | tr | — |
| L. cincinnatiensis, cluster 32 | 9.8 ± 1.7 | tr | tr | 15.8 ± 2.1 | 2.5 ± 0.2 | tr | — | — | 23.9 ± 1.8 | — | 18.6 ± 2.1 | tr | 7.4 ± 1.3 | — | — | tr | 3.6 ± 0.5 | 11.0 ± 2.5 | tr | tr | — |
| L. dumoffii | |||||||||||||||||||||
| Cluster 19 | 2.9 ± 0.6 | tr | tr | 37.2 ± 2.5 | tr | tr | — | — | 13.6 ± 1.1 | — | 13.1 ± 1.3 | tr | 3.7 ± 0.2 | — | — | — | 13.2 ± 0.7 | 4.8 ± 1.4 | 2.5 ± 0.9 | tr | — |
| Cluster 21 | 3.2 ± 0.9 | tr | tr | 32.4 ± 6.1 | tr | tr | — | tr | 14.6 ± 2.8 | — | 7.2 ± 2.0 | tr | 7.6 ± 3.6 | — | tr | — | 11.0 ± 0.8 | 11.6 ± 1.4 | 2.4 ± 0.8 | tr | tr |
| L. erythra and L. rubri-lucens, cluster 17 | 3.2 ± 0.6 | tr | tr | 14.1 ± 2.5 | tr | tr | — | tr | 29.9 ± 3.2 | — | 22.8 ± 3.7 | — | 7.8 ± 3.2 | tr | tr | tr | 10.2 ± 2.8 | — | tr | tr | tr |
| L. fairfieldensisT | 5.5 ± 0.3 | tr | tr | 10.8 ± 2.7 | tr | tr | — | tr | 37.7 ± 2.7 | — | 18.4 ± 2.1 | tr | 5.0 ± 0.5 | — | — | — | 3.3 ± 0.7 | 10.7 ± 2.0 | tr | tr | — |
| L. feeleii | |||||||||||||||||||||
| Cluster 15 | 5.6 ± 1.0 | tr | tr | 24.7 ± 2.3 | 3.1 ± 1.0 | tr | 2.8 ± 0.4 | — | 22.6 ± 2.6 | tr | 18.3 ± 1.9 | — | 7.5 ± 0.6 | — | — | tr | 6.0 ± 2.7 | tr | tr | tr | tr |
| Cluster 30 | 4.6 ± 1.1 | tr | tr | 20.6 ± 2.2 | 2.3 ± 0.6 | tr | 3.4 ± 1.4 | — | 17.3 ± 2.1 | 2.4 ± 0.4 | 23.8 ± 2.2 | — | 10.2 ± 2.0 | — | — | tr | 6.8 ± 0.5 | 3.4 ± 1.0 | tr | tr | — |
| L. geestianaT | tr | tr | 20.8 ± 0.5 | 9.2 ± 0.3 | tr | tr | — | — | tr | — | 33.0 ± 0.2 | — | 13.5 ± 1.6 | tr | — | 5.6 ± 0.6 | 2.8 ± 0.1 | — | tr | tr | — |
| L. genomospecies 1 | 2.8 ± 0.2 | tr | tr | 20.7 ± 1.3 | 4.5 ± 0.2 | tr | — | — | 21.9 ± 1.0 | — | 27.1 ± 0.8 | — | 8.0 ± 1.4 | — | — | tr | 7.8 ± 0.4 | — | tr | tr | — |
| L. gormanii | |||||||||||||||||||||
| Cluster 23 | 5.0 ± 0.6 | tr | tr | 24.5 ± 2.1 | tr | 3.0 ± 0.7 | — | — | 17.5 ± 2.2 | — | 10.7 ± 2.2 | tr | 8.2 ± 2.1 | — | — | tr | 8.9 ± 1.0 | 11.0 ± 1.9 | 2.6 ± 0.2 | tr | tr |
| ATCC 33297T | 7.9 ± 1.2 | tr | tr | 26.9 ± 3.1 | 4.1 ± 1.0 | 3.7 ± 0.7 | — | tr | 19.5 ± 2.9 | — | 8.1 ± 2.0 | tr | 4.8 ± 0.9 | — | — | tr | 6.6 ± 1.7 | 8.0 ± 1.0 | 2.3 ± 0.5 | tr | tr |
| L. gratianaT | 18.0 ± 0.8 | tr | tr | 14.8 ± 0.5 | 4.1 ± 0.2 | tr | — | tr | 26.1 ± 1.5 | — | 21.0 ± 2.8 | tr | 3.0 ± 0.4 | — | — | tr | tr | 4.0 ± 1.2 | tr | tr | — |
| L. hackeliae, cluster 8 | 3.3 ± 0.3 | 4.1 ± 1.9 | 3.5 ± 0.1 | 35.7 ± 2.8 | tr | tr | — | tr | 13.1 ± 1.5 | — | 12.2 ± 1.5 | — | 5.3 ± 2.2 | tr | — | tr | 8.4 ± 0.2 | 3.7 ± 0.0 | — | tr | — |
| L. israelensisT | 3.1 ± 0.4 | tr | tr | 37.9 ± 1.4 | tr | tr | — | 3.2 ± 0.2 | 19.4 ± 0.6 | — | 7.2 ± 0.1 | — | 4.8 ± 0.2 | tr | tr | tr | 11.2 ± 0.7 | 5.8 ± 0.2 | tr | tr | — |
| L. jamestowniensisT | tr | tr | tr | 39.0 ± 1.9 | tr | tr | — | 2.4 ± 0.1 | 11.8 ± 1.3 | — | 4.7 ± 0.4 | tr | 3.5 ± 0.2 | tr | 3.8 ± 0.2 | tr | 16.6 ± 1.3 | 5.8 ± 0.5 | tr | tr | — |
| L. jordanis, cluster 3 | 2.9 ± 0.1 | — | 2.3 ± 0.2 | 54.4 ± 2.1 | tr | tr | — | tr | 17.3 ± 0.9 | — | 2.6 ± 0.4 | tr | tr | tr | tr | tr | 11.1 ± 0.3 | 2.3 ± 0.8 | tr | tr | — |
| L. lansingensisT | tr | — | tr | 27.0 ± 0.4 | tr | tr | — | tr | 13.6 ± 1.6 | — | 3.1 ± 0.1 | tr | 3.5 ± 0.2 | tr | 4.5 ± 0.1 | 3.4 ± 0.2 | 33.7 ± 0.8 | tr | tr | tr | tr |
| L. londiniensis, cluster 18 | tr | tr | tr | 10.7 ± 1.9 | 3.7 ± 1.1 | tr | — | tr | 18.1 ± 3.3 | — | 20.2 ± 2.1 | — | 5.1 ± 2.1 | 3.1 ± 1.6 | — | 2.7 ± 1.4 | 21.6 ± 3.1 | — | 3.6 ± 0.8 | 2.5 ± 0.8 | tr |
| L. longbeachae, cluster 31 | 7.3 ± 0.9 | tr | tr | 14.8 ± 2.7 | tr | tr | — | tr | 23.1 ± 1.6 | — | 22.3 ± 2.6 | 3.6 ± 1.1 | 5.7 ± 1.7 | — | — | tr | 4.8 ± 0.7 | 9.6 ± 3.1 | tr | tr | — |
| L. maceachernii | |||||||||||||||||||||
| Cluster 7 | tr | — | — | 32.2 ± 0.6 | 3.1 ± 1.9 | tr | — | 2.4 ± 0.1 | 9.0 ± 0.4 | — | 17.4 ± 0.1 | — | 5.6 ± 0.4 | — | 5.5 ± 0.4 | — | 19.3 ± 1.5 | tr | tr | — | — |
| Toulouse20 n°5 | tr | tr | tr | 38.3 ± 1.7 | 4.4 ± 0.2 | 5.1 ± 0.7 | — | — | 6.3 ± 0.4 | — | 18.4 ± 0.8 | — | 9.2 ± 1.7 | — | — | — | 14.2 ± 0.7 | — | tr | tr | — |
| L. micdadei | |||||||||||||||||||||
| Cluster 5 | tr | tr | tr | 39.2 ± 1.8 | 2.4 ± 1.2 | 2.5 ± 0.9 | — | tr | 11.9 ± 1.5 | — | 8.2 ± 1.6 | tr | 4.9 ± 0.8 | — | 4.8 ± 0.7 | tr | 16.5 ± 2.6 | 2.8 ± 0.8 | tr | — | — |
| Cluster 6 | tr | tr | tr | 34.9 ± 1.8 | 5.8 ± 1.0 | 6.6 ± 0.6 | — | tr | 6.5 ± 1.1 | — | 14.6 ± 2.5 | — | 7.4 ± 0.4 | — | 5.4 ± 0.4 | — | 9.7 ± 1.5 | tr | tr | — | — |
| L. moravica, cluster 11 | 7.1 ± 0.9 | tr | — | 14.8 ± 5.3 | 2.7 ± 0.3 | tr | — | — | 21.9 ± 1.6 | — | 31.8 ± 5.4 | — | 7.0 ± 1.8 | — | — | — | 4.2 ± 1.8 | — | tr | 3.7 ± 0.1 | tr |
| L. nautarum, cluster 9 | 4.0 ± 1.7 | tr | tr | 38.1 ± 1.8 | 5.1 ± 1.6 | tr | — | — | 15.0 ± 3.9 | — | 16.5 ± 4.6 | tr | 5.3 ± 3.6 | — | — | — | 6.3 ± 0.4 | 2.4 ± 0.4 | — | — | — |
| L. oakridgensis, cluster 2 | tr | tr | — | 2.8 ± 0.8 | tr | tr | — | tr | 24.6 ± 3.6 | — | 18.8 ± 5.3 | tr | 10.4 ± 3.5 | tr | tr | tr | 5.9 ± 1.2 | 7.8 ± 4.8 | 2.8 ± 0.5 | 12.0 ± 1.4 | 2.5 ± 0.6 |
| L. parisiensis, cluster 25 | 3.2 ± 0.1 | tr | tr | 27.6 ± 2.7 | tr | tr | — | — | 15.5 ± 0.2 | — | 7.2 ± 1.0 | tr | 12.8 ± 0.5 | — | — | tr | 9.2 ± 0.2 | 11.6 ± 2.0 | 2.4 ± 0.3 | tr | tr |
| L. pneumophila, cluster 29 | 8.4 ± 1.0 | tr | tr | 15.3 ± 2.6 | 3.1 ± 0.6 | tr | — | 3.6 ± 1.0 | 32.8 ± 3.7 | — | 13.9 ± 3.9 | tr | 4.2 ± 1.5 | — | tr | tr | 5.1 ± 1.0 | 5.5 ± 2.5 | tr | tr | tr |
| L. quateirensisT | 7.9 ± 1.8 | tr | tr | 16.9 ± 2.4 | 4.4 ± 0.8 | tr | — | — | 17.6 ± 2.6 | — | 32.0 ± 1.6 | — | 5.1 ± 1.6 | — | — | tr | 4.4 ± 0.5 | — | tr | 3.2 ± 0.5 | tr |
| L. quinlivanii | |||||||||||||||||||||
| Cluster 14 | 2.6 ± 0.9 | tr | tr | 26.2 ± 0.8 | 6.0 ± 0.4 | 2.4 ± 0.8 | — | — | 20.2 ± 2.8 | — | 19.0 ± 0.8 | — | 6.2 ± 2.4 | — | — | tr | 11.3 ± 1.0 | — | tr | — | — |
| LC0870 | 2.3 ± 0.5 | tr | tr | 16.4 ± 0.7 | 4.2 ± 0.1 | tr | — | — | 20.4 ± 0.5 | — | 29.0 ± 0.8 | — | 9.0 ± 2.6 | — | — | tr | 9.8 ± 0.2 | — | 2.4 ± 0.2 | tr | — |
| L. sainthelensi, cluster 33 | 6.5 ± 0.9 | tr | tr | 12.0 ± 2.4 | 3.1 ± 0.7 | 2.3 ± 0.5 | — | tr | 20.6 ± 2.2 | — | 28.7 ± 2.6 | tr | 10.4 ± 2.0 | — | — | tr | 3.1 ± 0.6 | 8.4 ± 2.9 | tr | tr | — |
| L. santicrucisT | 14.8 ± 1.7 | tr | tr | 13.8 ± 0.9 | 2.9 ± 0.2 | tr | — | tr | 26.9 ± 0.8 | — | 24.4 ± 0.2 | — | 7.6 ± 1.5 | — | — | tr | 2.4 ± 0.3 | tr | tr | tr | — |
| L. shakespearei, cluster 28 | 14.8 ± 2.0 | tr | tr | 11.1 ± 0.9 | 6.1 ± 0.5 | 2.6 ± 0.8 | — | — | 36.5 ± 1.3 | — | 13.6 ± 2.4 | — | 4.1 ± 0.2 | — | — | tr | 2.4 ± 0.4 | — | 2.6 ± 1.1 | tr | tr |
| L. spiritensis, cluster 16 | 2.7 ± 0.6 | tr | tr | 20.6 ± 1.8 | tr | tr | — | 3.7 ± 2.1 | 30.0 ± 2.7 | — | 14.1 ± 0.9 | — | 5.2 ± 1.5 | tr | tr | tr | 14.6 ± 2.1 | tr | tr | tr | tr |
| L. steigerwaltiiT | 3.7 ± 0.2 | tr | tr | 26.6 ± 1.9 | tr | 3.4 ± 0.5 | — | — | 9.8 ± 1.7 | — | 7.4 ± 1.1 | tr | 20.7 ± 0.4 | — | — | tr | 4.8 ± 0.9 | 12.1 ± 0.6 | tr | tr | tr |
| L. tucsonensisT | 3.6 ± 0.2 | — | — | 34.6 ± 3.9 | tr | tr | — | tr | 16.6 ± 0.6 | — | 17.3 ± 0.7 | tr | 4.5 ± 0.2 | — | tr | tr | 11.6 ± 0.6 | 4.2 ± 1.6 | tr | tr | — |
| L. wadsworthiiT | tr | — | tr | 48.0 ± 0.9 | tr | tr | — | — | 13.9 ± 0.8 | — | 5.0 ± 0.2 | tr | tr | — | — | tr | 16.7 ± 1.0 | 5.8 ± 0.4 | tr | tr | — |
| L. waltersiiT | 22.6 ± 1.1 | tr | tr | 9.7 ± 0.7 | 9.1 ± 0.8 | 5.2 ± 1.1 | — | — | 21.2 ± 1.0 | — | 15.0 ± 0.5 | — | 7.1 ± 1.0 | — | — | tr | tr | — | tr | tr | tr |
| L. worsleiensis, cluster 12 | 7.4 ± 0.4 | tr | tr | 16.4 ± 1.2 | tr | tr | — | — | 18.8 ± 2.2 | — | 33.4 ± 0.6 | — | 9.6 ± 1.6 | — | — | — | 4.8 ± 1.6 | — | tr | tr | — |
| Legionella group I, cluster 1 | tr | tr | 36.7 ± 1.5 | 17.7 ± 1.4 | 9.1 ± 0.8 | 3.8 ± 0.2 | — | — | 3.8 ± 0.3 | — | 4.9 ± 0.6 | — | tr | tr | — | 6.5 ± 0.6 | 4.9 ± 0.1 | — | tr | — | — |
| Legionella spp. | |||||||||||||||||||||
| Montbeliard A1 | tr | tr | tr | 18.2 ± 1.6 | tr | tr | — | tr | 14.0 ± 0.9 | — | 34.5 ± 3.3 | — | 4.2 ± 0.2 | — | — | tr | 17.5 ± 1.2 | — | tr | tr | — |
| Greoux 11D13 | tr | tr | tr | 9.6 ± 0.3 | 3.6 ± 0.8 | tr | — | tr | 14.5 ± 1.5 | — | 44.4 ± 2.4 | — | 5.9 ± 1.6 | tr | — | tr | 7.1 ± 0.6 | — | tr | tr | tr |
| Legionella group II, cluster 22 | 2.6 ± 0.5 | tr | tr | 31.4 ± 4.5 | tr | tr | — | tr | 15.2 ± 2.8 | — | 8.1 ± 2.8 | tr | 7.3 ± 0.4 | — | tr | tr | 12.0 ± 1.4 | 9.8 ± 3.7 | tr | tr | tr |
a
The strains included in each cluster are shown in Table 1.
b
The fatty acids shown are those present in at least 2.0% of the total fatty acids. Values are means ± standard deviations.
c
The double-bond position indicated by the capital letter is unknown.
d
tr, fatty acid with less than 2.0% of the total.
e
—, fatty acid not detected.
f
Bold type indicates the major fatty acid of the unclustered strain or cluster of strains.