Figure 1.

Flowchart of cDDM creation pipeline for a couple of markers. (a) The acquired images are segmented in binary masks and their pixel connectivity separately explored by local density analysis for the two pseudo-color DDMs building. Then, the cDDM is built through local co-density analysis, by comparing the single markers DDMs pixelwise. (b) Details of local density (blue boxes) and co-density (orange boxes) analyses: after setting the search (moving) windows size, each foreground (FG) pixel of each binary mask is assigned a number representing the amount of FG pixels in its locality, this constituting the input to build the pseudo-color DDM (here shown with no “saturated” densities). Then, the local co-density analysis is performed by pixelwise subtraction of the two DDMs.