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. 2021 Oct 8;26(19):6079. doi: 10.3390/molecules26196079

Figure 3.

Figure 3

Evaluation of antagonistic effects induced by rubiscolins combined with positive control for MOR or KOR, observed using the CellKeyTM system. The cells expressing MOR (A) and KOR (B) were treated with each positive control alone or in combination with rubiscolin-5, rubiscolin-6, or 10−5 concentration of each negative control (10−11–10−5 M), and changes in impedance (ΔZiec) were measured using the CellKeyTM system. Concentration–response curves were prepared by calculating ΔZiec relative to the data obtained for each positive control: 10−5 M DAMGO for MOR (A) and 10−5 M U-50488H for KOR (B). All data points are presented as means ± S.E.M. for three independent experiments (n = 3–4).