FIG. 1.

The c-Myc N terminus recruits a HAT. (A) FLAG epitope-tagged GAL4 DBD or a FLAG epitope-tagged GAL4 DBD–c-Myc (amino acids 1 to 262) fusion was used as an affinity matrix to isolate proteins from HeLa cell nuclear extracts. (B) Proteins recruited by each matrix were incubated with purified histones in the presence of radiolabeled acetyl-CoA. Following filter binding and washing, the degree of histone acetylation in each sample was quantitated by scintillation counting. Assays were performed in triplicate. Values for individual samples after subtraction of background are reported.