FIG. 4.

The c-Myc oncoprotein associates with HAT activity in vivo. Immunoprecipitation of 293 cell lysates for either c-Myc, Max, or hGCN5 were subjected to an in vitro HAT assay in the presence of purified histones and radiolabeled acetyl-CoA. For each precipitation, lysate was prepared from a single confluent 15-cm-diameter dish of serum-stimulated 293 cells. Precipitations contained approximately 4 mg of total protein and were conducted in triplicate. Bars represent average values (± standard error).