FIG. 5.

In vivo association of hGCN5 and TRRAP in the absence of c-Myc. (A) Aliquots of 293 cell lysates were either electrophoresed directly (lane 1) or subjected to immunoprecipitation with nonimmune rabbit serum (NRS; lane 2) or anti-hGCN5 (lane 3). Proteins were resolved by SDS-PAGE (8% gel) and immunoblotted for TRRAP (upper panel) or hGCN5 (lower panel). Numbers at the left (in kilodaltons) indicate positions of molecular weight markers. (B) 293 cell lysates were subjected to immunoprecipitation with antibodies specific for hGCN5 (lane 2), c-Myc (lane 4), or species-matched, nonimmune sera (lanes 1 and 3). Precipitated proteins were resolved by SDS-PAGE (8% gel) and immunoblotted for c-Myc. The position of the heavy-chain polypeptide from the precipitating antibody is indicated (Ig).