A, RNA-seq data from multiple sources reveal Wnt ligand expression in the heart. GSE100532, GSE49906, and GSE1264381 from available datasets. CM-RNAseq was performed on isolated WT CMs, and Yap5SA nucRNA-seq was performed on PCM1-enriched nuclei from Yap5SA overexpression heart. B, tSNE visualization of graph-based clustering and classification for P6 neonatal hearts. C, The expression pattern of Wnt components in P6 hearts.