Fig. 5.

CV-MSC-mediated STAT3 activation increased autophagy in placental explants under hypoxic conditions. A Placental explants were treated with IL-6 or CV-MSC CM and with or without cryptotanshinone under hypoxic conditions, and p-JAK2, p-STAT3, P62 and LC3 expression levels were tested by western blotting analysis. Untreated placental explants under hypoxic conditions served as controls. B Placental explants were treated with IL-6 or CV-MSC CM and with or without cryptotanshinone under hypoxic conditions, and STAT3 and P62 expression levels were tested by immunohistochemistry. Untreated placental explants served as controls (scale bar, 50 μm). C Placental explants were treated with IL-6 or PE-CV-MSC CM and with or without cryptotanshinone under hypoxic conditions, and p-JAK2, p-STAT3, P62 and LC3 expression levels were tested by western blotting. Compared with A, PE-CV-MSC CM showed a lower effect of STAT3 and autophagy activation