Table 4.
Influence of rhizosphere compartment, crop species, and growth stage on entire and core bacterial microbiota
| Factor | Df | SumOfSqs | R2 | F | P* |
|---|---|---|---|---|---|
| Full dataset (close & distinct rhizosphere) | |||||
| Growth stage | 2 | 0.108 | 0.057 | 4.969 | < .001 |
| Crop species | 3 | 0.188 | 0.100 | 5.838 | < .001 |
| Rhizosphere (close&distant) | 1 | 0.425 | 0.225 | 39.53 | < .001 |
| Growth stage:crop species | 6 | 0.156 | 0.083 | 2.421 | < .001 |
| Residual | 94 | 1.01 | 0.535 | ||
| Core bacterial microbiota distant rhizosphere | |||||
| Growth stage | 2 | 0.081 | 0.020 | 8.398 | < .001 |
| Crop species | 3 | 3.694 | 0.918 | 253.680 | < .001 |
| Growth stage:crop species | 6 | 0.031 | 0.008 | 1.076 | 0.394 |
| Residual | 0.218 | 0.054 | |||
| Core bacterial microbiota close rhizosphere | |||||
| Growth stage | 2 | 0.119 | 0.030 | 8.842 | < .001 |
| Crop species | 3 | 3.384 | 0.851 | 166.968 | < .001 |
| Growth stage:crop species | 6 | 0.195 | 0.049 | 4.807 | < .001 |
| Residual | 41 | 0.277 | 0.070 | ||
| Bacterial microbiota distant rhizosphere | |||||
| Growth stage | 3 | 0.380 | 0.347 | 14.685 | < .001 |
| Crop species | 3 | 0.199 | 0.182 | 7.698 | < .001 |
| Growth stage:crop species | 6 | 0.127 | 0.116 | 2.454 | < .002 |
| Residual | 45 | 0.388 | 0.355 | ||
| Bacterial microbiota close rhizosphere | |||||
| Growth stage | 2 | 0.075 | 0.115 | 4.209 | < .001 |
| Crop species | 3 | 0.084 | 0.129 | 3.150 | < .001 |
| Growth stage:crop species | 6 | 0.126 | 0.195 | 2.370 | < .001 |
| Residual | 41 | 0.365 | |||
Based on permutational analysis of variance and weighted UniFrac distance
Core bacterial microbiota of wheat, barley, rye and oilseed rape includes ASVs present within each replicate per crop species and at every growth stage (booting, flowering, ripening)
*Bold font: factors considered significant