Fig. 8.

SIRT1 inhibitor EX527 abolished the anti-inflammatory effects of TC-G 1008 via GPR39/SIRT1/PGC-1α/Nrf2 signaling pathway at 48-h post-HIE. A Representative western blot bands. B–H Densitometric quantification of GPR39, SIRT1, PGC-1α, Nrf2, IL-1β, IL-6 and TNF-α in the ipsilateral hemisphere showed that EX527 significantly abolished the effects of TC-G 1008 treatment, resulting in a decrease in PGC-1α and Nrf2 expression, and an increase in IL-6, IL-1β and TNF-α expression.*p < 0.05 vs. sham; ^#p < 0.05 vs. HIE + vehicle; ^@p < 0.05 vs. HIE + TC-G1008 + control CRISPR, data are represented as mean ± SD, n = 6 for each group