Fig. 3.

Early detection model development. A, B Heatmap illustrating the cfDNA methylation levels for the selected DMRs between participants with ESCA, benign esophageal diseases, or healthy controls in the training (A) and test (B) sets. C, D Predicted probabilities of healthy control, benign esophageal diseases, and ESCA with different clinical stages in the training (C) and test (D) sets. E, F Receiver operating characteristic curve delineating the association between predictive probability and cancer in the training (E) and test (F) sets. G, H Sensitivity for ESCA with early and late stages in the training (G) and test (H) sets. Abbreviations: DMR, differentially methylated region; ESCA, esophageal cancer