TABLE 3.
Primers
| Gene | Primer | Sequence (5′→3′) | Product size (bp) |
|---|---|---|---|
| DIC | DIC-F | ATGGAGGCCCGAATTATGTCAGACAAAAGTGAATTAAAGG | 1,869 |
| DIC-R | TCGGTCGACCGAATTCTAAGCAGGTATTCGGGTAGC | ||
| DIC1–280 | DIC1–280aa-F | ATGGAGGCCCGAATTATGTCAGACAAAAGTGAATTA | 873 |
| DIC1–280aa-R | TCGGTCGACCGAATTTTAAGGGGCATCTTCATTGTTATT | ||
| DIC281–584 | DIC281–584aa-F | ATGGAGGCCCGAATTCATGAGCCTGATGGTGTGGCC | 945 |
| DIC281–584aa-R | TCGGTCGACCGAATTTTATTCATCATTGCGGGGAACAGC | ||
| NSP2 | NSP2-F | AGATTACGCTGAATTCGCTGAGCTAGCTTGCTT | 985 |
| NSP2-R | CGATGAGCTCGAATTTTAAATTCCGACATGTGAAACTTCA | ||
| NSP5 | HA-NSP5-F | AGATTACGCTGAATTCATGTCTCTCAGCATTGACGTA | 621 |
| HA-NSP5-R | CGATGAGCTCGAATTTTACAAATCTTCGATCAATTG | ||
| NSP5 | NSP5-F | CGCGGGCCCGGGATCCATGTCTCTCAGCATTGACGTA | 621 |
| NSP5-R | GCGACCGGTGGATCCAAATCTTCGATCAATTGCAT | ||
| VP6 | VP6-qPCR-F | CCACAATCTGAAGCACTAAGGA | 187 |
| VP6-qPCR-R | GCGCTGGCTGTGATCTATTC | ||