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. 2021 Oct 13;95(21):e00277-21. doi: 10.1128/JVI.00277-21

FIG 4.

FIG 4

Expression of lncRNA IFITM4P affects the mRNA levels of other IFITMs. (A) pNL-IFITM4P plasmid (0.5 μg) and plasmid (4.5 μg) carrying shRNA targeting either IFITM1, IFITM2, IFITM3, or IFITM4P were cotransfected into 293T cells. lncRNA IFITM4P levels were determined by RT-PCR 48 h posttransfection. (B and C) Knockdown (B) and overexpression efficiency (C) of lncRNA IFITM4P in A549 cells were assessed by RT-PCR. Western blotting was employed to assess the IFITM3 protein expression in lncRNA IFITM4P knockdown (B) and overexpressing (C) cells infected or not infected with WSN (MOI of 0.5). (D) lncRNA IFITM4P knockdown or control cells were infected or not infected with WSN for 14 h. The mRNA levels of IFITM1, IFITM2, and IFITM3 were then determined by qRT-PCR. (E) Samples of A549 cells overexpressing lncRNA IFITM4P were obtained as described for panel D. The mRNA levels of IFITM1, IFITM2, and IFITM3 were determined by qRT-PCR. Data represent mean values ± SEM (n = 3; *, P < 0.05; **, P < 0.01; ***, P < 0.001).