FIG 7.

Overexpression of IFITM1 or IFITM3 significantly upregulates the levels of lncRNA IFITM4P. (A to C) Overexpression efficiency of IFITM1 in A549 cells was determined by RT-PCR (A) and qRT-PCR (C). RT-PCR (A) was also employed to detect lncRNA IFITM4P expression in IFITM1-overexpressing and control cells with or without viral infection for 14 h. NP levels in panel A were quantitated by densitometry and normalized to GAPDH expression. The maximum value was regarded as 1 (B). (D to F) In A549 cells, the overexpression efficiency of IFITM3 was determined by Western blotting (D) and qRT-PCR (F). lncRNA IFITM4P expression in IFITM3-overexpressing and control cells infected or not infected with WSN for 14 h was determined by RT-PCR (D). NP levels in panel D were quantitated by densitometry and normalized to β-actin levels. The maximum value was regarded as 1 (E). (G and H) Levels of lncRNA IFITM4P in IFITM1 (G)- or IFITM3 (H)-overexpressing and control A549 cells infected or not infected with WSN were determined by qRT-PCR. (I to L) The mRNA levels of IFITM1 (J), IFITM2 (K), and IFITM3 (L) in IFITM4P-WT or IFITM4P-MU-miR-24-overexpressing and control A549 cells (I) with or without WSN infection (MOI of 0.5) were examined by qRT-PCR. Data represent mean values ± SEM (n = 3; *, P < 0.05; **, P < 0.01).