FIG 2.

Placenta histopathology is dependent on infecting virus strain. (A to C) The degree of placenta pathology was rated on a scale of 0 to 4, where 0 represents normal histologic features and 4 represents the most severe features observed. Each zone of the placenta was scored individually for general overall pathology, amount of inflammation, and amount of vascular injury. Only “general” scores are shown because they are representative of “inflammation” and “vascular injury” categories and do not differ significantly from “general.” Box and whiskers represent the minimum-to-maximum of all data points around the median. Data are representative of 3 to 6 animals per treatment group. ****, P ≤ 0.0001; **, P ≤ 0.01; *, P ≤ 0.05 (Kruskal-Wallis ANOVA). (D to G) Normal histologic features of each placental zone from PBS-inoculated dams. Labyrinth (L); junctional zone (JZ); decidua (D); normal blood vessel (BV) between the junctional zone and decidua. (H to K) Severe histopathologic injury patterns from placentas from ZIKV-CAM-inoculated dams. (H) Dilated vasculature (DV) and inflammation (I) in the labyrinth. (I) Thrombus (T) and inflammation at the junction of the junctional zone and decidua, and necrosis (N) in the decidua. (J) Necrosis and inflammation at the junction of the junctional zone and decidua, and necrosis of the decidua. (K) Dilated vascular space and inflammation in the labyrinth, hemorrhage (H) in the junctional zone, and necrosis in the decidua.