FIG 3.
Interferon-stimulated gene expression is elevated in placentas from ZIKV-infected dams. (A to C) RNA was extracted from placentas harvested on E14.5 and expression of the ISGs Oasl2 (A), Mx1 (B), and Ifit1 (C) were analyzed by qPCR. Levels were normalized to Gapdh and then ddCT was calculated relative to placentas harvested from PBS-inoculated dams. One to four placentas from two litters of PBS-inoculated and four to five litters of each ZIKV treatment group were analyzed. Means with standard deviation are shown. ***, P ≤ 0.001; **, P ≤ 0.01; *, P ≤ 0.05 (one-way ANOVA). (D to G) Spearman correlations with shaded 95% confidence interval are shown for % resorbed versus relative Mx1 expression (r = −0.6593; P value = 0.0169) (D), and 4 dpi maternal titer versus relative expression of Oasl2 (r = 0.3752; P value = 0.0171) (E), Mx1 (r = 0.4111; P value = 0.0084) (F), and Ifit1 (r = 0.3137; P value = 0.0487) (G).