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No robust evidence for type III IFN induction. RNA was extracted from placentas harvested on E14.5 and relative expression was analyzed by qPCR. Levels were normalized to Gapdh and then ddCT was calculated relative to placentas harvested from PBS-inoculated dams. (A) Ifnλr1 expression. (B) Spearman correlation of % resorbed and Ifnλr1 expression from individual pregnancies (r = −0.6099; P value = 0.0302). (C and D) Relative expression of IFNλ2 (C) and IFNλ3 (D).
