Figure 1.

Nampt overexpression ameliorates HFD-induced diastolic dysfunction. (A) HFD consumption upregulates Nampt. Heart lysates were prepared from NTg and Tg-Nampt mice after 3 months of HFD consumption. Western blot analyses were performed with indicated antibodies. (B) Body weights in NTg and Tg-Nampt mice after 3 months of HFD consumption. The mice were fed with HFD beginning at 6–12 weeks of age for 3 months. (C) Blood glucose in NTg and Tg-Nampt mice after 3 months of HFD consumption. (D) Glucose tolerance in NTg and Tg-Nampt mice under ND feeding and 3 months of HFD consumption. (E) Representative PV loop results after 3 months of HFD consumption. (F) Increased EDP and EDPVR were observed after 3 months of HFD consumption in NTg but were normalized in Tg-Nampt mice. (E-F) Mice were anesthetized using pentobarbital (G) LV fractional shortening was preserved in Tg-Nampt mice fed ND and after 3 months of HFD consumption. Statistical significance was determined with ANOVA (A and F (EDPVR)), repeated measures ANOVA (B, C, D (Left) and G) and the Kruskal-Wallis test (D (right) and F (EDP)).