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. Author manuscript; available in PMC: 2022 Jun 25.
Published in final edited form as: Circ Res. 2021 Apr 30;129(1):114–130. doi: 10.1161/CIRCRESAHA.120.317943

Figure 3.

Figure 3.

Nampt overexpression upregulates NAD(H) and NADP(H). (A) The levels of NAD+, NADH and total NAD++NADH were examined in Tg-Nampt mice after either normal diet or HFD consumption. (B) The levels of NADP+, NADPH, and total NADP++NADPH and the NADPH/NADP+ ratio were examined in Tg-Nampt mice under HFD feeding conditions. (C) Neither Nampt overexpression nor HFD significantly affects the level of NADK or G6PD (D) Nampt overexpression attenuates HFD-induced diastolic dysfunction in an NADK-dependent manner. Mice were anesthetized with Avertin. (E) Nampt overexpression attenuates HFD-induced cardiac hypertrophy in an NADK-dependent manner. (F) ThioN, an NADK inhibitor, inhibits Nampt-induced NADP(H), but not NAD(H) production. Statistical significance was determined with ANOVA (A-F).