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. 2021 Oct 4;148(19):dev199511. doi: 10.1242/dev.199511

Fig. 1.

Fig. 1.

The RpS5b mutant showed increased rRNA transcription. (A) Stage 7 egg chambers stained for Fibrillarin (red), Udd (green) and DNA (blue). RpS5b mutant nucleoli display elevated and more punctate Udd localization compared with control samples. Scale bar: 10 µm. (B) Western blots probed for Udd and β-Tubulin. The RpS5b mutant samples exhibit increased Udd levels. (C) Levels of Udd relative to β-Tubulin (n=3). Significance was determined using an unpaired Student's t-test (*P<0.1, ***P<0.001). (D) Schematic representation of ribosomal RNA (rRNA) processing. Probes used for northern blot analysis are color coded. (E) Northern blot analysis of wild-type and RpS5b mutant ovaries. 5S and ethidium bromide (EtBr) staining of mature 18S and 28S rRNA served as references. The RpS5b mutant ovaries expressed more pre-rRNA and intermediate processed rRNA transcripts compared with control samples. (F) Control and mutant egg chambers pulse labeled with Br-UTP for 5 min in the presence of α-amanitin stained for Br-UTP (red) and DNA (blue). Scale bar: 10 µm. (G) Quantification of immunofluorescence intensity. Significance was determined using an unpaired Student's t-test (***P<0.001).