Fig. 2.

Ribosome biogenesis is upregulated in the absence of RpS5b. (A) Schematic of the ribosome profiling experiment. (B) Gene ontology enrichment analysis of cellular components for upregulated transcripts. Ribosome-related genes (red bars) showed upregulated translational efficiency in RpS5b mutant ovaries compared with wild type. (C) Volcano plot of translational efficiencies among ribosomal proteins. Significantly changed genes are indicated as red dots. x and y axes are log₂ fold change (log₂FC) and negative value of log₁₀ P values (-log₁₀pvalue), respectively. (D) Representative ribosomal protein genes. Integrated genomics viewer (IGV) showing RNA-seq and ribosome footprint reads on indicated ribosomal proteins of control and RpS5b mutant samples.