Fig. 3.

Global translation rate is increased in the RpS5b mutant. (A) Western blot analysis of puromycin incorporation assay. Ponceau S staining was used as a loading control. RpS5b mutants have increased puromycin labeling, reflecting increased translation. (B) Plot of sucrose gradient fraction experiment showing UV absorbance at 254 nm of control (black line) and RpS5b mutant (red line) ovaries. Loss of RpS5b is accompanied by a modest increase in polysome peaks. (C) Box plots showing distribution of translation efficiencies in control and mutant samples. Significance was determined with the Wilcoxon signed rank test with continuity correction. ****P<2.2e-16.