Fig. 4.

RpS5b mutant germ cells display defects in yolk granule accumulation. (A) Volcano plot of translation efficiencies. Significantly changed genes are indicated with red dots. (B) Read maps of RNA-seq and footprints for Yolkless. (C) Translation efficiency of Yolkless. Significance was determined using the Benjamini and Hochberg method. ****P=2.75e-14. (D) DIC images of yolk granules in control w¹¹¹⁸ and RpS5b mutant oocytes. Stage 8 egg chambers in control ovaries start to accumulate yolk granules in the oocyte (arrow), but the mutant ovaries do not. Scale bars: 20 µm. (E) Auto-fluorescence images of yolk granules in control w¹¹¹⁸ and RpS5b mutant oocytes. Scale bars: 20 µm.