Fig. 5.

Posterior follicle cells in RpS5b mutant egg chambers display defects in Notch-Delta signaling. (A) Ovaries immunostained using anti-pH3 antibody (red) to label cells in mitosis. DNA was stained with DAPI (green). In control ovaries, follicle cells generally stop dividing at stage 6, but RpS5b mutant follicle cells still had pH3-positive cells at the posterior region after stage 7 (white arrows), indicating ongoing mitotic divisions. Scale bar: 20 µm. The images represent a stack of three optical slices. (B) Quantification of pH3-positive posterior follicle cells between control and RpS5b mutants (w¹¹¹⁸ n=84; RpS5b mutant n=76). (C) Ovaries immunostained using anti-Hnt antibody. Unlike control egg chambers, RpS5b mutant posterior follicle cells do not express Hnt (white arrows). Scale bar: 20 µm. (D) Ovaries immunostained using anti-Cut antibody. RpS5b mutant posterior follicle cells exhibit prolonged expression of Cut after stage 7 (white arrows). Scale bar: 20 µm.