Fig. 6.

Delta activation is disrupted in RpS5b mutant egg chambers. (A) Read maps of RNA-seq and footprints for Delta and Mindbomb1. (B) Ovaries immunostained using anti-Delta antibody. In control egg chambers, Delta accumulates at the cell junction between the oocyte and follicle cells (white arrow). By contrast, Delta does not localize to this junction and accumulates in the oocyte cytoplasm in the absence of RpS5b (arrowhead). Scale bars: 20 µm. (C,D) Ovaries were immunostained using anti-NICD (C) and anti-NECD (D) antibody. DNA is labeled with DAPI. Scale bars: 20 µm. (E) Ovaries were immunostained using anti-Mindbomb1 (Mib1) antibody (green). Mib1 localizes to the posterior region in control oocytes (arrow) but not in mutant oocytes (arrowhead). Scale bar: 20 µm.