Fig. 1.

VACV-driven protein expression. (a) Luciferase reporter plasmids containing the slp were transfected into HeLa cells which were subsequently infected with either the Copenhagen (wt) or NYVAC strains of VACV. Luciferase protein expression was measured via luciferase assay, and results made relative to those obtained from the wt-infected cells. (b) HeLa cells were transfected with luciferase reporter plasmids containing either the slp or hTEE-658 upstream of the coding region. Cells were then infected with either wt or NYVAC virus. Luciferase expression was determined by luciferase assay and results made relative to those obtained from cells containing the plasmid with slp, for the individual viruses. (c) Luciferase assay results from cells transfected with either an slp- or hTEE-658-containing plasmid, which were then infected with wt or NYVAC virus, respectively. Expression levels were made relative to those from the slp-plasmid transfected, wt-infected cells. (d) mRNA levels for cells transfected with either the slp- or hTEE-658-containing plasmids, and infected with either the wt or NYVAC virus, was determined via quantitative realtime PCR. Levels were made relative to those derived from cells transfected with the slp-containing plasmid in each pairing. All experiments were conducted in triplicate at minimum. Error reported as standard deviation.