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. 2021 Aug 12;102(8):001624. doi: 10.1099/jgv.0.001624

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© 2021 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution License.

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Fig. 3. — Combinatorial effect of translation enhancing elements. Luciferase production levels when reporter plasmids containing either hTEE-658 alone or in combination with a known translation enhancing element (TEE) were used in a transfect-infect assay with either the Copenhagen (a) or NYVAC (b) strain of VACV. Assays were conducted in HeLa cells, with luciferase assay results made relative to those when only hTEE-658 was present upstream of the luciferase coding region. All experiments were conducted in triplicate at minimum, and error is reported as standard deviation.