Fig. 4.

hTEE-658 driven expression of HIV-1 gp120. (a) Representative Western blot analysis of lysates from cells transfected with a plasmid containing either hTEE-658 alone or in combination with hTEE-675 or hTEE-884, and infected with the NYVAC strain of VACV. hTEE-658, or the indicated combination, was positioned within the plasmid immediately upstream of the coding region for the gp120 protein of HIV-1. Results of triplicate experiments are shown. (b) The normalized fluorescence of each band was determined, and then averaged for each sample. The averages were made relative to those when only hTEE-658 was present within the plasmid. (c) Representative Revert total protein staining of samples used for Western blot analysis. (d) Fluorescence of each Revert stained lane was determined, and then averaged for each sample. The averages were made relative to the sample with the highest average fluorescence. In all analyses, error is reported as standard deviation.