Fig. 5.

Recombinant virus containing hTEE-658. (a) Schematic outlining the use of homologous recombination to insert the firefly luciferase gene, either with or without hTEE-658 immediately upstream, into the TK locus of the NYVAC strain of VACV. In this strategy, the coding region for GFP is replaced with the inserted sequence of interest to aid in the detection of proper insertion. (b) Luciferase expression following infection of BSC40 cells with recombinant NYVAC. Luciferase assay results were made relative those when hTEE-658 was not included upstream of the luciferase coding region. Expression experiments were conducted in duplicate, with error reported as standard deviation.